Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYC

Cell type

Cell type Class
Neural
Cell type
ONS-76
Primary Tissue
Brain
Tissue Diagnosis
Medulloblastoma

Attributes by original data submitter

Sample

source_name
Medulloblastoma cell line
cell line
ONS76
engineered genotype
Inducible SOX2
antibody
MYC (Y69)

Sequenced DNA Library

library_name
GSM5662071
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked in 1% formaldehyde and subject to sonication. The resulting sheared chromatin was combined overnight with antibody. Libraries were prepared using NEBnext Ultra II DNA Library Prep Kit and NEBNext Multiplex Oligos for Illumina, following the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
143194865
Reads aligned (%)
97.3
Duplicates removed (%)
28.4
Number of peaks
15690 (qval < 1E-05)

hg19

Number of total reads
143194865
Reads aligned (%)
96.5
Duplicates removed (%)
29.0
Number of peaks
11942 (qval < 1E-05)

Base call quality data from DBCLS SRA